Fig. 1.

Antiviral activity of nitazoxanide (NTZ) against measles virus (MeV) and canine distemper virus (CDV). (A–B). MeV (A) and CDV (B) were incubated with the indicated concentrations of NTZ and added to Vero-hSLAM cells for 2 h to allow viral entry. After this time, infected cells were washed with PBS three times, and the medium containing carboxymethylcellulose was added. After 72 h, cells were fixed with 4% paraformaldehyde, and plaques were stained with crystal violet. Data represents the results from three independent experiments. (C–D) Vero-hSLAM cells were infected with 100 PFU/well of MeV (C) and CDV (D) viruses (96-well plate) and 2 h post-infection (p.i.), the medium was replaced with a complete medium containing different concentrations of NTZ. After 72 h, the supernatants were collected, and virus yield was determined by plaque assay. Data from three different experiments are shown. The error bars show the mean ± SEM.* p-value<0.05, **<0.01, ***<0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)