Fig. 2.

Effect of nitazoxanide (NTZ) on viral fusion and spread. (A) The fusion assay was performed in the presence or in the absence of NTZ (10 or 20 μg/ml) and in HEK-293T cells co-transfected with the measle virus (MeV) IC323–F-wt and MeV IC323–H-wt. Target cells were transfected with nectin-4 as a receptor. Effector cells were overlaid on target cells expressing the nectin-4 receptor and incubated overnight. NTZ was added to target cells 30 min before adding the effector cells. (B)PVero-hSLAM cells were infected with MeV IC323-EGFP in the presence of the indicated concentrations of NTZ. 72 h p.i the cells were fixed with 4 % paraformaldehyde, images were collected with a Nikon Ti2–U fluorescent microscope and the area of infection was measured in pixels using ImageJ software. (C) Graph showing the infection area in pixels measured with ImageJ software. * p-value<0.05.