TABLE 3.
Effects of glutamate, ammonium chloride, glucose, and/or potassium phosphate buffer on GS activity in SA mixturea
| Expt no. and additive(s) | mM additive | Relative activity (%) |
|---|---|---|
| 1, glutamate and ammonium chlorideb | 50, 50 | 91 |
| 100, 100 | 113 | |
| 200, 200 | 104 | |
| 300, 300 | 93 | |
| 400, 400 | 97 | |
| 50, 400 | 88 | |
| 400, 25 | 103 | |
| 2, glucose | 50 | 107 |
| 100 | 104 | |
| 150 | 100 | |
| 200 | 90 | |
| 3, phosphate buffer | 50 | 72 |
| 100 | 48 | |
| 150 | 33 |
Various concentrations of glutamate, ammonium chloride, and/or potassium phosphate buffer (pH 7.0) were added to SP mixture. GS activity is indicated relative to activity without the additive.
The two values are respective.