TABLE 4.
Activity of enzymes involved in glycolysis and gluconeogenesis in cell yeast strain extractsa
| Enzyme | Enzyme activity (μmol of product/min/mg of protein)
|
|
|---|---|---|
| Wild type | O-21 | |
| Hexokinase (glucose) | 1.55 (100) | 1.64 (106) |
| Hexokinase (fructose) | 1.31 (100) | 1.41 (108) |
| Glucose 6-phosphatase | 0.047 (100) | 0.037 (80) |
| Glucose 6-phosphate dehydrogenase | 0.249 (100) | 0.232 (95) |
| Phosphoglucomutase | 0.198 (100) | 0.113 (57) |
| Phosphoglucoisomerase | 2.56 (100) | 3.17 (123) |
| Phosphofructokinase | 0.108 (100) | 0.124 (115) |
| FBPase | 0.0075 (100) | 0.0014 (19) |
a
The activity of hexokinase was determined with glucose or fructose as a substrate. The values in parentheses indicate the activities relative to the activities in the cell extract of the wild-type strain.