REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
E. coli Trelief 5α	Tsingke Biological Technology	Cat.#TSC01
Chemicals, peptides, and recombinant proteins
Dulbecco’s modified Eagle’s medium	Corning	Cat.#10-013-CVRC
Fetal bovine serum	Sigma-Aldrich	Cat.#F8687
Trypsin-EDTA	Sigma-Aldrich	Cat.#SM-2003
PolyJet transfection reagent	SignaGen Laboratories	Cat.#L100688
Annexin V Alexa Fluor 647	Thermo Fisher Scientific	Cat.#R37175
Sytox Green	Thermo Fisher Scientific	Cat.#S7020
CaCl2	Sigma-Aldrich	Cat.#C5670
Critical commercial assays
Endo-Free plasmid DNA kit	OMEGA	Cat.#D6950-02
Experimental models: Cell lines
HEK293T	ATCC	Cat.#CRL-3216
Recombinant DNA
pmCherry-N1-GSDME-NT	This paper	N/A
Software and algorithms
Zen	Zeiss	http://www.zeiss.com
Other
LSM710 confocal microscope system equipped with environmental control chamber	Zeiss	http://www.zeiss.com
Steri cycle CO2 incubator	Thermo Fisher Scientific	https://www.thermofisher.com/
NanoPhotometer N60	Implen Spectrophotometer	https://www.implen.de/
Eppendorf 5424	Eppendorf	https://www.eppendorf.com
35-mm glass-bottom dish	Corning	Cat.#801002
T-25 flask	Corning	Cat.#3289