Lung digesting medium
| Reagent | Final concentration | Amount |
|---|---|---|
| HBSS (with Ca2+ and Mg2+) | 99% | 100 mL |
| Collagenase | 1 mg/mL | 0.5 mL |
| DNase I | 10 U/mL | 0.2 mL |
The lung digesting medium has to be prepared fresh every time.
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