| Experimental models: Cell lines |
|
| MC38 GFP-labeled cells |
Giannou et al.1
|
N/A |
| MC38 shC |
Giannou et al.1
|
N/A |
|
| Experimental models: Organisms/strains |
|
| C57BL/6J wild-type mice, adult, 24–30 g BW, used as recipient animal |
The Jackson Laboratory |
N/A |
| Knockout mouse for the respective structure of interest, e.g., Il22ra1−/− mice on a B6 background, adult, 24–30 g BW, used as donor animal |
Till Strowig Lab – Helmholtz Center for Infection Research, Braunschweig, Germany |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| PBS |
Dirk Hindorf Anprotec |
AC-BS-0002 |
| FBS |
Capricorn Scientific |
CS-HI-1A |
| EDTA |
Carl Roth GmbH |
8043.2 |
| DMEM (GlutaMAX) |
Gibco |
31966047 |
| Penicillin/Streptomycin |
Gibco |
15070-063 |
| Metamizole |
Ratiopharm GmbH |
9051799 |
| HBSS (with Ca2+ and Mg2+) |
Life Technologies GmbH |
14065-049 |
| Collagenase |
Sigma |
C2139-5G |
| DNase I |
AppliChem über Th. Geyer |
A3778-50MG |
| 0.05% Trypsin-EDTA |
Gibco |
25300-054 |
|
| Other |
|
| Leica M620 TTS tabletop surgical microscope |
Leica Microsystems |
|
| Induction chamber for small animals |
UNO Roestvaststaal BV |
180000232 |
| Ventilator for small animal use, e.g., UMV-03 UNO microventilator |
UNO Roestvaststaal BV |
180000023 |
| Isoflurane vaporizer |
UNO Roestvaststaal BV |
180000008 |
| Flowmeter CM2 |
UNO Roestvaststaal BV |
180000002 |
| Artificial lung |
UNO Roestvaststaal BV |
180000009 |
| Heating plate |
UNO Roestvaststaal BV |
180000028 |
| Control unit 01 for heating plate |
UNO Roestvaststaal BV |
180000123 |
| Laboratory stand with clamp, e.g., Wisamic lab stand |
Amazon |
18-01-22-0001-EU |
| Small surgical scissors, e.g., fine scissors, sharp |
Fine Science Tools |
14060-09 |
| Small blunt surgical scissors, e.g., Strabismus scissors |
Fine Science Tools |
14074-09 |
| Small surgical forceps, e.g., Mueller micro forceps, straight, 110 mm |
Braun Aesculap |
FM001R |
| Curved surgical forceps, e.g., Student fine forceps, curved |
Fine Science Tools |
91117-10 |
| Fine angled forceps, e.g., Dumont #5/45 forceps |
Fine Science Tools |
11251-35 |
| Fine spring scissors, straight, e.g., micro scissors, straight, sharp/sharp, 105 mm |
Braun Aesculap |
OC496R |
| Fine spring scissors, curved, e.g., micro scissors, curved, sharp/sharp, 105 mm |
Braun Aesculap |
OC297R |
| Micro needle holder, e.g., Mueller micro needle holder, curved, 180 mm |
Braun Aesculap |
FM061R |
| Adjustable helping hand tool |
Toolcraft |
ZD-10F |
| Retractor, e.g., Alm retractor (self-retaining), 70 mm, 4 × 4 prongs, semi-sharp |
Braun Aesculap |
BV010R |
| Vascular clips, e.g., Biemer micro vascular clip, oblique serrated, 5 mm opening width, 9 mm |
Braun Aesculap |
FD562R |
| Small electrocautery, e.g., cautery high temp 2″ extended shaft loop tip |
Bovie Medical |
AA05 |
| LOOK 6-0 silk suture spool, black braid, 100 yards |
Corza Medical |
SP114 |
| Dafilon 10-0/30 cm/DLM6 |
B. Braun |
G1118749 |
| Prolene 5-0/90 cm/2xRB1 |
B. Braun |
8556H |
| Vasofix Safety, 20 G, PUR |
B. Braun |
4269110S-01 |
| Vasofix Safety, 22 G, PUR |
B. Braun |
4269098S-01 |
| Versatus-W, winged i.v. catheter, 26 G |
Terumo |
SR + DU2619WX |
| Sandpaper, 240 grit |
BAHAG AG |
N/A |
| Injekt Solo, 10 mL syringe |
B. Braun |
4606108V |
| Injekt F, 1 mL syringe |
B. Braun |
9166017V |
| Berpu cannula 25 G, 30 mm |
B. Braun |
BP2530430 |
| Venofix Safety 21 G, 30 cm length |
B. Braun |
4056504-01 |
| Tape, e.g., Durapore surgical tape |
3M |
1538-1 |
| Gauze compresses, 5 × 5 cm |
Fuhrmann GmbH |
31501 |
| Petri dishes, e.g., Sterilin Petri dishes |
Thermo Fisher Scientific |
501V |
| Cotton swabs, wood, 15 cm |
NOBA Verbandmittel |
10859 |
| Ointment, e.g., Bepanthen Augen- und Nasensalbe |
Bayer Vital GmbH |
N/A |
| Isofluran-Piramal (isoflurane) 250 mL |
Piramal Critical Care BV |
N/A |
| Temgesic Ampullen (buprenorphine hydrochloride, 0.3 mg/mL) |
Indivior Europe Ltd. |
N/A |
| Thiopental Inresa 0.5 g (sodium thiopental) |
Inresa Arzneimittel GmbH |
N/A |
| Heparin-Natrium-25000-ratiopharm (sodium heparin, 25,000 IU/5 mL) |
Ratiopharm |
N/A |
| Preservation fluid (Perfadex Plus) |
XVIVO Perfusion |
N/A |
| Neubauer hemocytometer (cell counting) |
Neubauer |
68052-14, 68052-15 |