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. 2023 Nov 10;4(4):102701. doi: 10.1016/j.xpro.2023.102701
REAGENT or RESOURCE SOURCE IDENTIFIER
Experimental models: Cell lines

MC38 GFP-labeled cells Giannou et al.1 N/A
MC38 shC Giannou et al.1 N/A

Experimental models: Organisms/strains

C57BL/6J wild-type mice, adult, 24–30 g BW, used as recipient animal The Jackson Laboratory N/A
Knockout mouse for the respective structure of interest, e.g., Il22ra1−/ mice on a B6 background, adult, 24–30 g BW, used as donor animal Till Strowig Lab – Helmholtz Center for Infection Research, Braunschweig, Germany N/A

Chemicals, peptides, and recombinant proteins

PBS Dirk Hindorf Anprotec AC-BS-0002
FBS Capricorn Scientific CS-HI-1A
EDTA Carl Roth GmbH 8043.2
DMEM (GlutaMAX) Gibco 31966047
Penicillin/Streptomycin Gibco 15070-063
Metamizole Ratiopharm GmbH 9051799
HBSS (with Ca2+ and Mg2+) Life Technologies GmbH 14065-049
Collagenase Sigma C2139-5G
DNase I AppliChem über Th. Geyer A3778-50MG
0.05% Trypsin-EDTA Gibco 25300-054

Other

Leica M620 TTS tabletop surgical microscope Leica Microsystems
Induction chamber for small animals UNO Roestvaststaal BV 180000232
Ventilator for small animal use, e.g., UMV-03 UNO microventilator UNO Roestvaststaal BV 180000023
Isoflurane vaporizer UNO Roestvaststaal BV 180000008
Flowmeter CM2 UNO Roestvaststaal BV 180000002
Artificial lung UNO Roestvaststaal BV 180000009
Heating plate UNO Roestvaststaal BV 180000028
Control unit 01 for heating plate UNO Roestvaststaal BV 180000123
Laboratory stand with clamp, e.g., Wisamic lab stand Amazon 18-01-22-0001-EU
Small surgical scissors, e.g., fine scissors, sharp Fine Science Tools 14060-09
Small blunt surgical scissors, e.g., Strabismus scissors Fine Science Tools 14074-09
Small surgical forceps, e.g., Mueller micro forceps, straight, 110 mm Braun Aesculap FM001R
Curved surgical forceps, e.g., Student fine forceps, curved Fine Science Tools 91117-10
Fine angled forceps, e.g., Dumont #5/45 forceps Fine Science Tools 11251-35
Fine spring scissors, straight, e.g., micro scissors, straight, sharp/sharp, 105 mm Braun Aesculap OC496R
Fine spring scissors, curved, e.g., micro scissors, curved, sharp/sharp, 105 mm Braun Aesculap OC297R
Micro needle holder, e.g., Mueller micro needle holder, curved, 180 mm Braun Aesculap FM061R
Adjustable helping hand tool Toolcraft ZD-10F
Retractor, e.g., Alm retractor (self-retaining), 70 mm, 4 × 4 prongs, semi-sharp Braun Aesculap BV010R
Vascular clips, e.g., Biemer micro vascular clip, oblique serrated, 5 mm opening width, 9 mm Braun Aesculap FD562R
Small electrocautery, e.g., cautery high temp 2″ extended shaft loop tip Bovie Medical AA05
LOOK 6-0 silk suture spool, black braid, 100 yards Corza Medical SP114
Dafilon 10-0/30 cm/DLM6 B. Braun G1118749
Prolene 5-0/90 cm/2xRB1 B. Braun 8556H
Vasofix Safety, 20 G, PUR B. Braun 4269110S-01
Vasofix Safety, 22 G, PUR B. Braun 4269098S-01
Versatus-W, winged i.v. catheter, 26 G Terumo SR + DU2619WX
Sandpaper, 240 grit BAHAG AG N/A
Injekt Solo, 10 mL syringe B. Braun 4606108V
Injekt F, 1 mL syringe B. Braun 9166017V
Berpu cannula 25 G, 30 mm B. Braun BP2530430
Venofix Safety 21 G, 30 cm length B. Braun 4056504-01
Tape, e.g., Durapore surgical tape 3M 1538-1
Gauze compresses, 5 × 5 cm Fuhrmann GmbH 31501
Petri dishes, e.g., Sterilin Petri dishes Thermo Fisher Scientific 501V
Cotton swabs, wood, 15 cm NOBA Verbandmittel 10859
Ointment, e.g., Bepanthen Augen- und Nasensalbe Bayer Vital GmbH N/A
Isofluran-Piramal (isoflurane) 250 mL Piramal Critical Care BV N/A
Temgesic Ampullen (buprenorphine hydrochloride, 0.3 mg/mL) Indivior Europe Ltd. N/A
Thiopental Inresa 0.5 g (sodium thiopental) Inresa Arzneimittel GmbH N/A
Heparin-Natrium-25000-ratiopharm (sodium heparin, 25,000 IU/5 mL) Ratiopharm N/A
Preservation fluid (Perfadex Plus) XVIVO Perfusion N/A
Neubauer hemocytometer (cell counting) Neubauer 68052-14, 68052-15