FIG. 1.

(A through E) RAPD patterns obtained with the GeneAmp model 2400 thermal cycler and primers B10, B12, A15, B18, and A1, respectively, by using DNAs from various strains of N. fowleri. With these five primers, the three Taq DNA polymerases that we used produced similar discriminating fragments. The Taq DNA polymerase from Gibco-BRL was used for the gels shown in panels A and D, and the Taq DNA polymerase from Appligene was used for the gels shown in panels B, C, and E. The arrows indicate the positions of the additional amplified fragments. (A) All of the strains except the Australians strains produced the 1,580-bp additional fragment generated with primer B10. Lane 1, NH1; lane 2, Morgan; lane 3, ORAM; lane 4, PA90; lane 5, MW4u; lane 6, J26(50)45E; lane 7, Na1165b; lane 8, Ch2-1-e13b; lane 9, RC-5; lane 10, L2-1-d11; lane m, molecular size marker (123-bp DNA ladder). (B and C) Lane 1, NH1; lane 2, Morgan; lane 3, ORAM; lane 4, MW4u; lane 5, PA105; lane 6, Ch2-1-e13b; lane 7, Na1165b; lane 8, J16(1)42E; lane 9, J26(50)45E; lane 10, G1-3-f18; lane 11, RC-5; lane 12, EjI-SON; lane 13, 124; lane 14, Enterprise; lane 15, TY; lane 16, CJ; lane 17, EPA 988s-GP2; lane 18, WM; lane 19, MCM; lane 20, L2-1-d11. (B) When primer B12 was used, in addition to the 930-bp fragment, one specific band at 510 bp differentiated the widespread variant from the other variants. (C) When primer A15 was used, the Euro-American variant was distinguishable from the other variants since it did not produce the 615-bp additional fragment. (D and E) Lane 1, NH1; lane 2, Morgan; lane 3, Ch2-1-e13b; lane 4, J16(1)42E; lane 5, J26(50)45E; lane 6, Na1165b; lane 7, 124; lane 8, EPA 988s-GP2; lane 9, 123-bp DNA ladder. (D) When primer B18 was used, the two Japanese strains and the French strain from Cattenom were the only strains which produced the 310-bp additional fragment. (E) Only one of the two Japanese strains produced the strongly amplified 600-bp fragment obtained with primer A1.