Fig. 8.

Metformin inhibits microglial activation in ACC of AR mice through NLRP3/IL-1β pathway. A Representative immunofluorescence images of colocalization of NLRP3 in microglia in ACC. B Immunofluorescence of intensity of IBA-1. C Representative western blot analysis of TET2, NLRP3, Caspase-1, and ASC protein in the ACC from three groups. D Normalized expression of TET2, NLRP3, Caspase-1, and ASC protein in the ACC by western blot analysis. E, F Levels of IL-1β and IL-18 were determined by ELISA in the ACC. G Levels of 5-hmC determined by dot blot in the ACC. H Quantitative analysis of 5-hmC levels in the ACC from three groups. Data are presented as mean ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001)