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. 2023 Nov 27;27:122. doi: 10.1186/s40824-023-00448-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Effects of successive mild HT on human AD-MSCs during subculturing with HT. a Schematic representation of the experimental study design. b Metabolic activity, c cumulative cell number, and d doubling time of the MSCs in each group during the subculture. e Telomere lengths of the MSCs at P + 10(HT) in each group. Relative gene expressions of f HSPs (HSP27, HSP70, and HSP90) and g stemness markers (SOX2, OCT4, and NANOG) at P + 1(HT), P + 4(HT), P + 7(HT), and P + 10(HT) in each group. Gene expression levels were normalized with those of the control at each passage. h Populations and i MFI of the positive marker-stained MSCs in each group. *p < 0.05 compared to the control group, and ^#p < 0.05 compared to the HT 41ºC group