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. 2023 Nov 27;21:452. doi: 10.1186/s12951-023-02110-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Increased permeability, decreased coverage, and disrupted TJs of BSCB after SCI. A Representative images of the spinal cord following EB injection 7 days after spinal cord injury. B Quantitative measurement of EB content in spinal cord injury areas. C, D Detection of TJs protein expression and quantification at different time points after SCI by immunoblotting. E Representative immunostaining of CD31, Pdgfrβ and TJs markers in the spinal cord injury area at different time points after SCI. F, G Expression levels and quantification of hypoxia-related proteins in spinal cord injury regions at different time points after SCI detected by immunoblotting