Fig. 1. Generation & characterization of Slc1a4^E256K (Slc1a4^K/K) mutant mice.

(A) Schematic of the Slc1a4 locus, showing sequence of exon 4 in the region targeted by CRISPR/Cas9 gene editing. The sgRNA binding site is indicated by the green arrow and the cytosine mutated to adenine is shown in red. (B) Chromatograms from Sanger sequencing of PCR products spanning the region targeted by CRISPR/Cas9 gene editing from wildtype (top), heterozygous (middle) and homozygous mutant (bottom) mice showing the G to C change corresponding to position 978 of the cDNA sequence. (C-D) Western blot analysis showed similar expression of SLC1A4 in the brains of wildtype (Slc1a4^E/E) and Slc1a4^K/K mutant mice. Relative expression of SLC1A4 normalized to b-tubulin-III. SLC1A4 expression was not significantly different between wildtype and Slc1a4^K/K mutant mice, and was not detected (ND) in the null mutants.