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. Author manuscript; available in PMC: 2024 Dec 1.
Published in final edited form as: Mamm Genome. 2023 Aug 29;34(4):572–585. doi: 10.1007/s00335-023-10013-4

Figure 4. The p.E256K mutation alters the kinetics of D-serine uptake.

Figure 4.

(A) Wildtype or p.E256K mutant human SLC1A4 was expressed in Xenopus oocytes and [3H]-labeled D-serine uptake measured by liquid scintillation spectroscopy. The mutant transporter consistently showed increased affinity for D-serine (Km) but lower maximum velocity (Vmax), indicating saturation at a lower concentration of D-serine. (B) Brain slices from Slc1a4K/K mice showed higher [3H]-labeled D-serine (100 nM) uptake than Slc1a4E/E (wildtype) brain slices, consistent with the mutant transporter change in D-serine kinetic parameters.