TABLE 1.
Results of insect feeding experiment with tobacco bud worm comparing insecticidal activities of Cry1Ac protein and Cry1Ac-expressing phage
| Protein or phage | LC50a (95% CIb) | LC50a (pmol of Cry1Ac) |
|---|---|---|
| Cry1Ac protein | 7.6 (3.5–13.7) ng | 11.7 |
| 1Ac-fUSE5 phage | 7.0 (4.2–11.0) × 108 TU | 11.2 |
| 1Ac-Kpn-fUSE5 phage | 10.7 (7.2–15.8) × 108 TU | 17.1 |
| Wild-type phage | None detectedc | Not applicable |
a
As determined by the probit analysis (29). All doses are expressed on a per well basis and were surface applied (approximate surface area, 1.8 cm2 per well.
b
CI, confidence interval.
c
Forty insects fed 1010 TU of WT-fUSE5 each (14 times the LC50 of 1Ac-fUSE5) showed 0% mortality and 2.5% growth inhibition.