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Competition binding of 125I-radiolabelled phage to BBMV
| Radiolabelled toxin or phage added to 10 μg of BBMV | 100-fold excess of unlabelled Cry1Ac | cpm added | Mean cpm bound (n = 2) | % cpm bound | % Compe- tition |
|---|---|---|---|---|---|
| 125I-Cry1Ac protein | − | 98,242 | 12,690 | 12.9 | 66.5 |
| + | 98,242 | 4,256 | 4.3 | ||
| 125I-1Ac-fUSE5 phage | − | 105,252 | 12,293 | 11.7 | 8.5 |
| + | 105,252 | 11,212 | 10.7 | ||
| 125I-WT-fUSE5 phage | − | 62,319 | 3,784 | 6.1 | None |
| + | 62,319 | 4,743 | 7.6 |
