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[Preprint]. 2024 Mar 25:2023.11.13.566830. Originally published 2023 Nov 13. [Version 2] doi: 10.1101/2023.11.13.566830

Figure 4. Gorilla CD4 variants differentially support entry of SIVcpz.

Figure 4.

(A) HIV-1ΔEnv-GFP viruses were pseudotyped with Envs (top of graphs) from diverse SIVcpz strains. Cf2Th cells stably expressing human CCR5 and various CD4s (X-axis) were infected with various volumes of these pseudoviruses and then analyzed by flow cytometry 48 hours post infection. GFP positive cells were enumerated and virus titers (transducing units per milliliter; TDU/mL) were determined for those samples falling within the linear infection range (n = 2 titration points). The mean virus titers obtained from each of three independent experiments were plotted (dots), with error bars representing the standard error of the mean (SEM). (B) Data from each pseudotyped Env in A were used to calculate virus titer means normalized to cells expressing human CD4 and were plotted as a heat map, where red and white represent susceptibility or resistance to viral entry, respectively.