Fig 1. Individual Septins are recruited to the wound edge.
(A) Drosophila Septins and their respective classes. (B) Potential Drosophila Septin hetero-hexamers. (C-H’) Confocal max projection images of embryos expressing an actin reporter (sGMCA or sStMCA; C-C’) and Sep1-GFP (D-D’), Sep4-GFP (E-E’), ChFP-Pnut (F-F’), Sep2-GFP (G-G’), or Sep5-RFP (H-H’), at the time points indicated. (C’-H’) Kymographs across the wound area in C-H, respectively. (I-M) Fluorescence intensity (arbitrary units) profiles across the wound area over time for the images shown in (C-H). Scale bars: 20μm.
