Fig 2. Spatial recruitment of individual Septins is class independent.
(A-E) Confocal max projection images of embryos co-expressing an actin reporter (sGMCA or sStMCA) and Sep1-GFP (A), Sep2-GFP (B), ChFP-Pnut (C), Sep4-GFP (D), or Sep5-RFP (E). Scale bars: 20μm. (A’-E’) Orthogonal cross-sectional image of A-E, respectively. (F-J) Normalized fluorescence intensity (arbitrary units) average profiles (for n≥10) depicting localization of individual Septins with respect to the actin ring. Peak expression is indicated by the respective arrowheads.
