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[Preprint]. 2023 Nov 16:2023.11.14.567084. [Version 1] doi: 10.1101/2023.11.14.567084

Fig 5. Septin complexes crosslink actin filaments into curved bundles, whereas individual Septins bundle but do not bend actin filaments.

Fig 5.

(A-R) in vitro generated actin filaments in the presence of no added protein (A-B), Sep1/Sep2/Pnut complex (C-D), Sep4/Sep5/Pnut complex (E-F), Sep1 alone (G-H), Sep2 alone (I-J), Pnut alone (K-L), Sep4 alone (M-N), Sep5 alone (O-P), or Sep1 + Sep2 + Sep4 proteins (Q-R). Circular structures formed in the presence of the Sep1/Sep2/Pnut or Sep4/Sep5/Pnut complexes are indicated by red arrowheads. (S) Quantification of actin circles per field of view formed by the individual or complexes of Septins as indicated. (T) Quantification of the area of the actin circles per field of view formed by each of the two Septin complexes as indicated. P value and number of structures assayed (n=) are indicated. (U) Coomassie stained gel of the purified Sep1/Sep2/Pnut and Sep4/Sep5/Pnut complexes. Septin proteins are indicated: 1=Sep1, 2=Sep2, 4=Sep4, 5=Sep5, P=Pnut. Scale bars: 20 µm. Error bars represent ±SEM. Kruskal-Wallis test was performed for S; Mann-Whitney test was performed for T. Comparison to control is indicated by asterisks: *** p<0.001, **** p<0.0001, ns is not significant.