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[Preprint]. 2023 Nov 16:2023.11.14.567084. [Version 1] doi: 10.1101/2023.11.14.567084

Fig 6. Anillin is required for optimal wound repair.

Fig 6.

(A-C’) Confocal max intensity projection images of embryos expressing an actin marker (sStMCA; A), GFP-Anillin (B), or both (C). (A’-B’) Kymographs across the wound area in A-B, respectively. (C’) Orthogonal cross-sectional image of an embryo expressing sStMCA and GFP-Anillin. (D) Fluorescence intensity (arbitrary units) average profile (for n=10) depicting recruitment of Anillin with respect to the actin ring. Peak expression is indicated by the respective arrowheads. (E) Confocal max projection images of wound generated in embryos expressing an actin marker (sGMCA) in an Anillin RNAi background. (E’) Kymograph across the wound area depicted in E. Wound expansion is highlighted by yellow lines. Actin recruitment to the actomyosin ring is indicated by yellow arrows. Delayed actomyosin ring disassembly is indicated by red arrowheads. Delay in wound closure is indicated by blue arrowheads. Scale bars: 20μm. (F) Quantification of the wound area over time for knockdown shown in (E). (G-J) Quantification of fold wound expansion (G), wound contraction rate (H), actin ring width (I), and actin ring intensity (J). Black line and error bars represent mean ± SEM. Red dotted line and square represent mean ± 95% CI from control. Kruskal-Wallis test: * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001, ns is not significant. Scale bars: 20 µm.