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[Preprint]. 2023 Nov 16:2023.11.14.567084. [Version 1] doi: 10.1101/2023.11.14.567084

Fig 7. Anillin functions upstream of Sep1 and Sep2, but not Sep4, Sep5, or Pnut.

Fig 7.

(A-C) Confocal max projection images of wounds generated in embryos expressing an actin reporter (A-C) and GFP-Anillin (A’-C’) in Sep1 RNAi knockdown (A-A’), Sep4 RNAi knockdown (B-B’), or Pnut RNAi knockdown (C-C’) backgrounds. (D) Quantification of GFP-Anillin recruitment (area under the curve of fluorescence profile) in control, Sep1 RNAi, Sep4 RNAi, and Pnut RNAi backgrounds at 50% wound closure. (E-I) Confocal max projection images of wounds generated in embryos expressing an actin reporter (E-I) and Sep1-GFP (E’), Sep2-GFP (F’), ChFP-Pnut (G’), Sep4-GFP (H’), or Sep5-RFP (I’) in an Anillin RNAi knockdown background. (J) Quantification of Sep1-GFP, Sep2-GFP, ChFP-Pnut, Sep4-GFP, and Sep5-RFP recruitment (fluorescence intensity) in control and Anillin RNAi backgrounds at 50% wound closure. Error bars represent ±SEM. Kruskal-Wallis test was performed for D and J. Comparison to control is indicated by blue asterisks: * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001, ns is not significant. Scale bar: 20 µm.