Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| iTaq Universal SYBR Green Supermix | Bio-Rad | Cat# 1725120 |
| iScript™ gDNA Clear cDNA Synthesis Kit | Bio-Rad | Cat# 1725034 |
| Na2ATP | Sigma-Aldrich | Cat# A2383 |
| Methyl Cellulose | Sigma-Aldrich | Cat# M0512 |
| Protocatechuate Acid (PCA) | Sigma-Aldrich | Cat# 03930590 |
| Protocatechuate 3,4-dioxygenase (PCD) | Sigma-Aldrich | Cat# P8279 |
| Trolox | Sigma-Aldrich | Cat# 238813 |
| Amyl Acetate | Electron Microscopy Sciences | Cat# 10815 |
| Sterile Collodion (Parlodion) in 2% Amyl Acetate | Electron Microscopy Sciences | Cat# 12620–10 |
| Rhodamine Muscle Actin | Cytoskeleton, Inc. | Cat# AR05 |
| GDP | Sigma-Aldrich | Cat# G7252 |
| GMP-PNP | Sigma-Aldrich | Cat# G0635 |
| GST-Rho1 | PMID:16518391 | N/A |
| GST-Rac1 | PMID:16518391 | N/A |
| GST-Cdc42 | PMID:16518391 | N/A |
| Experimental models: Organisms/strains | ||
| sGMCA (GFP actin reporter) | PMID:10769037 | N/A |
| sStMCA (mScarlet actin reporter) | Bloomington Drosophila Stock Center | BDSC_90928 |
| UASp-Sep1-GFP | Bloomington Drosophila Stock Center | BDSC_51346 |
| Sep2-GFP | Bloomington Drosophila Stock Center | BDSC_26257 |
| UASp-mCh-Pnut | Provided by T. Lecuit | PMID:23410938 |
| UASp-Sep4-GFP | Bloomington Drosophila Stock Center | BDSC_51345 |
| UASp-Sep5-RFP | Provided by A. Wilde | N/A |
| UASp-GFP-Anillin | Bloomington Drosophila Stock Center | BDSC_51348 |
| TRiP.HMC03041 (Vermilion RNAi) | Bloomington Drosophila Stock Center | BDSC_50641 |
| UASz-sep1-shRNA | This study | N/A |
| Sep2[2] | Provided by D Clark | PMID:24433211 |
| TRiP.HMC05924 (Pnut RNAi) | Bloomington Drosophila Stock Center | BDSC_65157 |
| TRiP.GL01287 (Sep4 RNAi) | Bloomington Drosophila Stock Center | BDSC_41859 |
| Sep5[2] | Provided by D Clark | PMID:24433211 |
| TRiP.GL01269 (Ani RNAi) | Bloomington Drosophila Stock Center | BDSC_41841 |
| matalpha4-GAL-VP16 | Bloomington Drosophila Stock Center | BDSC_7063 |
| Oligonucleotides (5' -> 3') | ||
| qPCR-Sep1-F: CCAACCAGGTGCATCGAAAGT | This study | N/A |
| qPCR-Sep1-R: CAGCTTTACGGTTTGCTTTTGTT | This study | N/A |
| qPCR-Sep4-F: AGCGCATCGAGAAGACGAC | This study | N/A |
| qPCR-Sep4-R: CTGTCCTCACAGTTGATGGCA | This study | N/A |
| qPCR-Pnut-F: CATCTACAACGCGGAACAGTAT | This study | N/A |
| qPCR-Pnut-R: ACCGTGAGTGTCAGATTGACG | This study | N/A |
| qPCR-Ani-F: CTTTCCCAACGTAACGACCTT | This study | N/A |
| qPCR-Ani-R: GAATCTCAACTTGCACCCCAA | This study | N/A |
| qPCR-RpL32-F: ATGCTAAGCTGTCGCACAAATG | PMID:33306674 | N/A |
| qPCR-RpL32-R: GTTCGATCCGTAACCGATGT | PMID:33306674 | N/A |
| Sep1-shRNA-top: ctagcagtGCATTATTCCGGACGCCATAGtagttatatt caagcataCTATGGCGTCCGGAATAATGCgcg | This study | N/A |
| Sep1-shRNA-bot: aattcgcGCATTATTCCGGACGCCATAGtagttatattc aagcataCTATGGCGTCCGGAATAATGCactg | This study | N/A |
| Recombinant DNA | ||
| pCite4-Ani-RBD | This study | N/A |
| pETDuet-Sep1-Sep2 | This study | N/A |
| pETDuet-Sep4-Sep5 | This study | N/A |
| pET28-dt-Pnut | This study | N/A |
| pGEX-dt-Sep1 | This study | N/A |
| pGEX-dt-Sep2 | This study | N/A |
| pGEX-dt-Pnut | This study | N/A |
| pGEX-dt-Sep4 | This study | N/A |
| pGEX-dt-Sep5 | This study | N/A |
| Software and algorithms | ||
| Fiji | PMID:22743772 | http://fiji.sc/ |
| R | StatSoft | https://www.r-project.org/ |
| Matlab | Mathworks | http://mathworks.com/ |
| GraphPad Prism | GraphPad | https://www.graphpad.com/ |
| Other | ||
| Revolution WD spinning disk confocal microscope | Andor | N/A |
| Micropoint | Andor | N/A |
| CFX96TM Real Time PCR Detection System | Bio-Rad | N/A |
| Fred Hutchinson Cancer Center – Leica Center of Excellence | Fred Hutchinson Cancer Center | RRID:SCR_02272 0 |
| Fred Hutchinson Cancer Center Cellular Imaging Core Facility | Fred Hutchinson Cancer Center | RRID:SCR_02260 9 |