Figure 1: Cryo-EM structures of hBest1 in the open and “partially open” states.

A Open state hBest1 (PDB ID 8D1O) and D partially open state hBest1 (PDB ID 8D1K) structures visualized with ion permeation pathway in white. The transmembrane neck region of the B open state and E partially open state structures, with ion permeation pathway colored by hydrophobicity with pale brown corresponding to maximum hydrophobicity and green corresponding to maximum hydrophilicity. Top-down view of the C open state neck and F partially open state neck. G Pore radius profiles of the open, partially open and closed (PDB ID 8D1I) states.