FIG. 2.

Analysis of protein components of the insecticidal toxin complex of P. luminescens. (A) SDS–10% PAGE analysis of HPLC-purified P. luminescens insecticidal toxin preparations. The gel was stained with Coomassie brilliant blue. Sta, size standard; tox, purified toxin sample. (B) HPLC-purified toxin separated by native agarose gel electrophoresis; 5 μg of total protein was loaded. The gel was stained with Coomassie brilliant blue. (C) SDS–18% PAGE analysis of protein bands separated by native agarose gel electrophoresis. Lanes: 1, band 1; 2, band 2; 3, band 3. The gel was silver stained. (D) HPLC-purified toxin separated by agarose gel electrophoresis in the presence of 0.1% Triton X-100; 4 μg of total protein was loaded. The gel was stained with Coomassie brilliant blue. (E) SDS–10% PAGE analysis of protein bands separated in native agarose gels in the presence of 0.1% Triton X-100. Lanes: 1, band 1; 2, band 2; 3, band 3. The positions of molecular mass markers are indicated.