TABLE 3.

Toxicities of injected or orally administered HPLC-purified toxin from P. luminescens W-14 for fourth-instar M. sexta larvae

Samplea	% Mortalityb	Initial wt (g) ± SE	Final wt (g) ± SE	Fold weight gainb
Fedc
Control buffer	0	0.23 ± 0.02	6.15 ± 0.48	26.7
0.2 μg	0	0.26 ± 0.02	2.74 ± 0.50	11.0
2.0 μg	0	0.25 ± 0.03	0.68 ± 0.14	2.7
20 μg	75	0.24 ± 0.03	0.20 ± 0.01	0.8
Injectedd
Control buffer	0	0.56 ± 0.02	8.02 ± 0.30	14.4
0.05 μg	58	0.52 ± 0.03	1.02 ± 0.07	1.9
0.5 μg	100	NA	NA	NAe
5 μg	100f	NA	NA	NA

^an = 12 larvae for each sample group. 

^bAt 6 days posttreatment. 

^cToxin was applied to 1 cm² of diet in 50 μl of KPO₄ buffer, pH 6.9. 

^dToxin was injected in 5 μl of KPO₄ buffer, pH 6.9. 

^eNA, not applicable (all larvae died). 

^fAll larvae were dead by 24 h postinjection.