TABLE 4.
Enzymatic activities of culture broth versus HPLC-purified P. luminescens insecticidal toxin
| Activity | Activity ofa:
|
|
|---|---|---|
| Culture broth | HPLC-purified toxin | |
| Protease | 0.68 | 0 |
| Lipase | ||
| Ca precipitate | + | − |
| Colorimetric | 5.5 | 0.2 |
| Hemolysin | + | − |
| Phospholipase C | + | − |
| Nuclease | + | − |
a
+, activity present; −, no activity present. Units of activity for protease: change in optical density of an azocasein solution, 2 h; units of activity for lipase (colorimetric): micromoles of p-nitrophenol liberated from p-nitrophenyl palmitate in 30 min.