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. 2023 Nov 13;20(3):e20220030. doi: 10.1590/1984-3143-AR2022-0030

Table 1. Summary of use of nanoparticles in reproduction.

Nanoparticle used Effect Reference
1 Iron oxide nanoparticles (IONPs)-antiubiquitin antibodies (Abs) complex Successful depletion of dead/damaged spermatozoa from buffalo (Bubalusbubalis) semen Bisla et al. (2021)
2 Magnetic nanoparticles (MNPs) based sexing in donkeys Highly effective in selecting X spermatozoa, without affecting several physiological sperm parameters Domínguez et al. (2018)
3 Magnetic iron oxide nanoparticles (MNPs) Magnetic tools (MNP) improve fertility in magnetic assisted cell-sorting or MACS as an effective method Durfey et al. (2017); Farini et al. (2016)
4 Zinc oxide nanoparticles
for cryopreservation
Higher percentage of total and progressive motility in frozen-thawed samples in ZnONPs group compared to control. Chromatin damage and malondialdehyde (MDA) level was significantly lower Isaac et al. (2017)
5 Silver nanoparticles Effective antimicrobial agent in porcine sperm to replace conventional antibiotics Pérez‐Duran et al. (2020)
6 Zinc nanoparticles Increase mitochondrial activity, enhances membrane integrity, reduce lipid peroxidation, improves total antioxidant capacity in bovine spermatozoa Jahanbin et al. (2021)
7 Cerium oxide (CeO2) Motility parameters of semen improved after 48 h of incubation.
Integrity of plasma membranes of spermatozoa maintained
Falchi et al. (2018)
8 Selenium nano-particles
(Se-NPs)
1.0 μg/ml improved post-thaw sperm quality of Holstein bulls, reduced apoptosis, lipid peroxidation and sperm damage that occurs during cryopreservation. Khalil et al. (2019)
9 Silver-carbon NPs
(Ag@C NPs)
Efficient antimicrobial activity (Minimum Inhibitory Concentration: 3.125–12.5 μg/mL) against the E. Coli, S. aureus, P. aeruginosa and strong bactericidal effect on S. aureus, and P. aeruginosa (Minimum Bactericidal Concentration: 3.125 μg/mL), with no detrimental effect on the percentage of sperm motility plasma membrane integrity, acrosome integrity and normal sperm morphology at concentrations of 15 and 30 μg/mL, respectively, after a cold storage of 48 h. Yousef et al. (2021)
10 Green synthesized gold nanoparticles (GSGNPs, 5; 10ppm/mL) Increased motility, livability, and membrane integrity post-equilibration, improved in Tris-extended samples in post-equilibrated and post-thawed semen.
Viable sperm increased, early apoptotic, apoptotic, and necrotic sperm decreased @10 ppm GSGPNs.
Ismail et al. (2020)