Table 4. Effect of silver nanoparticles on the motility and abnormality parameters before and after freezing.
| Parameter | Control |
Group 1
(NP- 125 µg/ml) |
Group 2
(NP- 250 µg/ml) |
p value | |
|---|---|---|---|---|---|
| Motile (%) | Before Freezing | 61.3 ± 2.81 | 66.25 ± 2.70 | 63.7 ± 2.04 | 0.392 |
| After Freezing | 36.95 ± 1.81 | 41.83 ± 1.42 | 39 ± 1.31 | 0.085 | |
| Progressive motile (%) | Before Freezing | 44.4 ± 2.68 | 48.2 ± 3.06 | 46.45 ± 1.94 | 0.589 |
| After Freezing | 28.35b ± 1.84 | 35.99a ± 1.40 | 32.1ab ± 1.77 | 0.009 | |
| Motile Conc. (Million/ml) | Before Freezing | 17.15 ± 2.69 | 17.75 ± 3.06 | 18.5 ± 2.60 | 0.943 |
| After Freezing | 7.68 ± 0.92 | 10.94 ± 1.02 | 8.51 ± 1.21 | 0.087 | |
| Bent Tail Million/ml | Before Freezing | 0.00 | 0.00 | 0.00 | - |
| After Freezing | 0.18 ± 0.08 | 0.39 ± 0.23 | 0.35 ± 0.14 | 0.06 | |
| Coiled Tail | Before Freezing | 0.00 | 0.00 | 0.00 | - |
| After Freezing | 0.0055 ± 0.005 | 0.007 ± 0.005 | 0.01 ± 0.005 | 0.830 | |
| Distal droplet | Before Freezing | 0.00 | 0.00 | 0.00 | - |
| After Freezing | 1.13 ± 0.14 | 1.43 ± 0.19 | 1.20 ± 0.28 | 0.579 | |
| DMR % | Before Freezing | 1.15 ± 0.18 | 1.05 ± 0.15 | 1.20 ± 0.19 | 0.826 |
| After Freezing | 1.14 ± 0.14 | 1.43 ± 0.19 | 1.20 ± 0.28 | 0.595 |
Mean values with different superscript in a row differ significantly (p<0.05).