Skip to main content
. 2023 Oct 23;51(21):11439–11452. doi: 10.1093/nar/gkad900

Figure 6.

Figure 6.

L-Apt12-6 can regulate gene activity in cells. (A) Schematic illustration of the design of luciferase reporter plasmids. The wildtype or mutant c-KIT construct is inserted into the HSV-TK promoter of Firefly luciferase. (B) Normalized luciferase activity of wildtype c-KIT plasmid (WT) decreases with addition of 0 nM, 100 nM, 200 nM and 300 nM L-Apt12-6. (C) Normalized luciferase activity of mutant c-KIT plasmid (Mut) has no obvious change with addition of L-Apt12-6. (D) Relative luciferase mRNA expression levels of WT decrease with addition of L-Apt12-6. (E) Relative luciferase mRNA expression levels of Mut have no obvious change with addition of L-Apt12-6. (F) Western blotting shows endogenous c-KIT expression in HGC-27 cells decreases with L-Apt12-6 treatment. (G) Western blotting shows endogenous c-KIT expression in HGC-27 cells decreases with PDS treatment. (H) Western blotting shows endogenous c-KIT expression in HGC-27 cells has no obvious change with L-SL1 treatment. The Western blotting results are quantified by ImageJ. (I) QPCR result shows relative c-KIT mRNA expression level normalized to house-keeping gene GAPDH decreases with L-Apt12-6 treatment. (J) Schematic illustration of L-Apt12-6 binding to c-KIT promoter G4 blocks c-KIT gene transcription and translation. The error bar represents the SEM of three independent replicates. *P< 0.05, **P< 0.01, ***P< 0.001, ns: not significant.