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. 2023 Sep 18;228(11):1610–1620. doi: 10.1093/infdis/jiad391

Figure 2.

Figure 2.

MAL-I binds to sialylated gonococcal LOS. A, Schematic of Neu5Ac addition to the terminal lactosamine of gonococcal LOS. MAL-I binding site is indicated. B, MAL-I binding to Neisseria gonorrhoeae F62 wild type following bacterial incubation with increasing concentrations of CMP-Neu5Ac, presented as MFI. Data are from a single experiment, with 3 biological replicates per CMP-Neu5Ac concentration. Error bars represent standard deviation. Abbreviations: CMP-Neu5Ac, cytidine-5′-monophospho-N-acetyl neuraminic acid; Gal, galactose; GlcNAc, N-acetylglucosamine; LDmanHep, L-glycero-D-manno-heptose; Kdo, 2-keto-3-deoxy-D-mannooctanoic acid; LOS, lipooligosaccharide; Lst, LOS-sialyltransferase; MAL-I, Maackia amurensis lectin I; MFI, median fluorescence intensity.