Extended Data Fig. 7. Dimerization is needed for BBε CAR surface expression.
a, Schematic representation of monomeric and dimeric BBε CARs and the mutations introduced. ERR, endoplasmic reticulum retention signal. b,c, Flow cytometric analysis (b) and quantification (c) of surface CAR expression for GFP+ or BFP+ cells (n = 3). d, Specific killing of CD19+ Nalm6 cells by CAR T cells (1:1 ratio) for 12 h. (n = 3). e, f, Cytokine secretion, IFN-γ (e) and TNF (f), assayed by ELISA of CAR T cells upon incubation with Nalm6 (1:1 ratio) for 24 h (n = 3). g, Flow cytometric analysis and h, quantification of surface CAR expression for BFP+ or GFP+ cells (n = 3). Each dot represents an independent donor. Data are represented as mean ± s.d. Two-tailed paired t-test (c–f). One-way ANOVA followed by Holm-Sidak’s multiple comparisons test (h).