Extended Data Fig. 8. Gating strategy for flow cytometric analysis.

a, Gating strategy to sort for GFP⁺ cells as presented for Fig. 1b. b Gating strategy to gate on phenotypic T cell subsets as presented in Fig. 2b. c Gating Strategy to determine CD107a upregulation on CAR T cells upon Nalm6 co-culture as presented in Fig. 2g. d, Gating Strategy for Fig. 4a. e, Gating Strategy for Fig. 5a. f, Gating strategy to determine GFP⁺CAR⁺ or BFP⁺CAR⁺ T cell populations as presented for Fig. 6b, Extended Data Figs. 7 and g. g, Gating strategy to determine BFP⁺CAR⁺ T cell populations as presented for Fig. 7b.