Fig. 7. The enhanced functionality of the BBε CAR relies on motifs recruiting Lck.
a, Schematic representation of the mutations introduced in BBε. b,c, Flow cytometric analysis (b) and surface CAR expression (c) gated on BFP+ cells (n = 5). d, Specific killing of CD19+ Nalm6 cells by CAR T cells (1:1 ratio for 6–8 h). Each dot represents an independent donor (n = 5 for BBε, n = 4 for BBεΔBRS and BBεΔPRS, n = 3 for BBεΔRK, BBεΔPRSΔRK and BBεΔBRSΔRK, and n = 2 for BBεΔITAM). Data are represented as mean ± s.d. One-way ANOVA followed by Dunnett’s multiple-comparison test was used. e, Percentage of CAR T cells fluxing calcium after contacting SLBs functionalized with adhesion and costimulatory molecules as well as CD19 molecules at indicated densities. Data represent one out of three independent experiments done with three independent donors. f, Percentage of CAR T cells positive for activation markers after 48 h of stimulation with CD19+ Nalm6 cells (1:1 ratio). Two representative donors are shown.