Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 2;24(12):2068–2079. doi: 10.1038/s41590-023-01661-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 3 — (a) Confocal microscopy images show the intracellular localization and accumulation of SARS-CoV-2 nonstructural protein 3 (NSP3) and double-stranded RNA (dsRNA) in BALF CD64⁺ Mac isolated from WTM and OM at ≥221 days p.i. After 8 hours of culture, cells were fixed with 4% formaldehyde. Immunostaining included DAPI (nucleus; purple), anti-dsRNA (red), and anti-NSP3 (green). Yellow arrows highlight dsRNA within the macrophages. (b) Similar to (a), these confocal microscopy images depict the intracellular localization of NSP3 and dsRNA in BALF CD64⁺ Macs, with the addition of Phalloidin (white) staining to highlight protrusions within the macrophages. Blue arrows indicate the presence of protrusions. Increased threshold on fields displaying only Actin was used to facilitate visualization of pseudopod- and TNT-like structures. (c) Confocal microscopy images as in (b) show syncytia observed in MAC culture. Scale bars in (a) = 45 µm, Scale bars in (b) and (c) = 35 µm. Some images use 3D rendering techniques to highlight the three-dimensional aspect of the visualization.