Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 2;24(12):2068–2079. doi: 10.1038/s41590-023-01661-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — a, Histogram showing the MFI of MHC-E in K562 cells transduced with MHC-E (MHC-E*01:03) after peptide loading (VL9, HSP60, V_3–11) and culture for 12 h (left), and graph illustrating the expression of MHC-E in MHC-E*01:03 cells after peptide loading (with VL9, HSP60, and V_3–11 peptides) compared to the control condition with no peptides, following a 12-h culture (right). b, Histogram displaying the MFI of biotinylated peptides (VL9, HSP60, V_3–11) loaded into MHC-E*01 cells and revealed through conjugation with streptavidin, following a 12-h culture period (left) and expression of biotinylated peptides (VL9, HSP60, V_3–11) in MHC-E*01:03 cells after peptide loading (with VL9, HSP60 and V_3–11 peptides), in comparison to the control condition with no peptides, following a 12-h culture period (right). c, Dot plot illustrating the distribution of blood NK cells from 13 human PBMCs collected before 2019 based on NKG2A and NKG2C expression (left) and frequency of NKG2C⁺NKG2A⁻, NKG2C⁺NKG2A⁺ and NKG2C⁻NKG2A⁺ cell subsets in human PBMCs collected before 2019 (right). d, Inhibition of degranulation in human NKG2C⁺NKG2A⁻, NKG2C⁺NKG2A⁺, NKG2C⁻NKG2A⁺ and NKG2C⁻NKG2A⁻ NK cell subsets exposed to K562-E-01 cells loaded with VL9 and HSP60 peptides during an 8-h coculture, as in c. e, Inhibition of degranulation in human NKG2C⁺NKG2A⁻, NKG2C⁻NKG2A⁺ and NKG2C⁺NKG2A⁺ NK subsets during 8-h coculture with VL9, HSP60 and the spike-derived peptide V_3–11. f, Inhibition of degranulation in BALF NKG2R⁺ NK cells isolated ≥461 d p.i. from 15 WTM and incubated with V_3–11 peptide-loaded K562-E-01 cells for 8 h. g, Spearman correlation analysis between V_3–11 peptide-induced inhibition of NKG2R⁺ NK cell degranulation and viral load measured in Fig. 2a, Spike⁺ Mac frequency measured in Fig. 2c and GzmB expression in NKG2R^hi NK cells in total BALF cells of WTM after 24 h of spike stimulation, as measured in Fig. 7b. Each symbol represents an individual; bars represent medians. Interquartile ranges are shown. In all graphs, P values were determined by Kruskal–Wallis test with Dunn’s post hoc test.

Source data