Figure S2. H3K4me3 and H3K36me3 are altered upon demethylase deletion but not always associated with gene expression changes.

(A) Overlap between the DMGs and the DEGs. For H3K36me3, only 25 genes were overexpressed upon increase in H3K36me3 after RPH1 deletion. Similarly, for the H3K4me3 modification, the increase in methylation was accompanied by a significant increase in expression for only 17 genes. (B, C) Metagene plots depict the binned median signal from transcription start sites to transcription end sites of all genes for H3K36me2, H3K36me3, H3K4me3, and input from BY4741 WT, (B) BY4741 jhd2Δ strains, and (C) rph1Δ strains. Increase in the H3K36me3 and H3K4me3 methylation is observed for both mutants, whereas a decrease or no change is observed for the H3K36me2 signal.