Fig. 3.

Rps9 D95N mutation has no detectable effect on levels of phosphorylated Tau in the cortex of mice with different humAPP alleles. a Representative western blot images and densitometry analysis of Tau and phosphorylated Tau proteins, comparing humAPP^WT/WT/Rps9 WT with humAPP^WT/WT/Rps9 D95N mice (10 ≤ N ≤ 12) (Males N = 6; Females 4 ≤ N ≤ 6). GAPDH was used as loading control. Densitometry depicts relative expression of target gene normalized to loading control. For comparison, the non D95N genotype was set as 1. N = number of mice in each comparison. Graph shows mean, SEM and individual data points. b Representative western blot images and densitometry analysis of Tau and phosphorylated Tau proteins comparing humAPP^{NL−F/NL−F}/Rps9 WT with humAPP^{NL−F/NL−F}/Rps9 D95N mice (9 ≤ N ≤ 11) (Males 4 ≤ N ≤ 6; Females N = 5). GAPDH was used as loading control. Densitometry depicts relative expression of target gene normalized to loading control. For comparison, the non-D95N genotype was set as 1. N = number of mice in each comparison. Graph shows mean, SEM and individual data points