Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 14;11:1272667. doi: 10.3389/fcell.2023.1272667

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Battaglia, Sacco, Vecchio, Scicchitano, Petriaggi, Giorgio, Bulotta, Levi, Faniello, Biamonte and Costanzo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Treatment with ferlixit leads to ferroptosis in 3D HEY tumor spheroids. (A) PI flow cytometric analysis of 3D HEY and PEO1 tumor spheroids untreated and treated with 100 μM ferlixit. % of dead cells (PI positive) are reported in each plot. Relative histogram is reported on the right. Flow cytometry analysis of mitochondrial ROS levels (B) and lipid peroxidation (C) quantified by using MitoSOX and BODIPY-C11 reagent, respectively, in 3D HEY and PEO1 tumor spheroids after administration of 100 μM ferlixit. Relative histograms are reported on the right. (D) Western blot of VDAC2 and GPX4 in 3D HEY and PEO1 cells upon administration of 100 μM ferlixit. γ-TUB was used as loading control. Results were obtained from three independent experiments.