Table 2.
A panel of miRNAs tested as potential controls for RT-qPCR-based assessment of miRNA levels in human blood plasma.
| miRNA | Sequence | |
|---|---|---|
| hsa-miR-23a-3p | AUCACAUUGCCAGGGAUUUCC | Haemolysis controls |
| hsa-miR-451a | AAACCGUUACCAUUACUGAGUU | |
| hsa-miR-16-5p | UAGCAGCACGUAAAUAUUGGCG | |
| Spike-In cel-miR-39-3p | UCACCGGGUGUAAAUCAGCUUG | Exogenous controls (isolation control) |
| Spike-In cel-miR-54-3p | UACCCGUAAUCUUCAUAAUCCGAG | |
| Spike-In cel-miR-2-3p | UAUCACAGCCAGCUUUGAUGUGC | Exogenous controls (cDNA synthesis control) |
| Spike-In cel-miR-238-3p | UUUGUACUCCGAUGCCAUUCAGA | |
| hsa-miR-93-5p | CAAAGUGCUGUUCGUGCAGGUAG | Endogenous controls (normalisers) |
| hsa-miR-192-5p | CUGACCUAUGAAUUGACAGCC | |
| hsa-miR-24-3p | UGGCUCAGUUCAGCAGGAACAG | |
| hsa-miR-126-3p | UCGUACCGUGAGUAAUAAUGCG | |
| hsa-miR-16-5p | UAGCAGCACGUAAAUAUUGGCG | |
| hsa-miR-484 | UCAGGCUCAGUCCCCUCCCGAU | |
| hsa-miR-23a-3p | AUCACAUUGCCAGGGAUUUCC |