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. Author manuscript; available in PMC: 2023 Nov 28.
Published in final edited form as: Neurobiol Dis. 2022 Dec 14;176:105964. doi: 10.1016/j.nbd.2022.105964

Figure 3: The non-canonical pathway of the inflammasome is upregulated in the hippocampus of 16 months-old Epm2b−/− mice.

Figure 3:

(A) Schematic representation of the canonical (left) and non-canonical (right) inflammasome pathways. (B and C) Quantification of the intensity signal detected by immunofluorescence in the RLM+MoDG area of 16 months-old Epm2b−/− and wt mice for caspase-11 (B) or GSDM-D (C). Results are expressed as median with a range of four to six different samples from each genotype, including male and female mice. (D) Protein levels of caspase-11 (cleaved and full-length protein), GSDM-D, caspase-1, and NLRP3 proteins, were analyzed in hippocampi extracts from 16 months-old wt (C1-C3) and Epm2b−/− (M1-M3) mice by western blot using the indicated antibodies. Molecular weight standards are on the right. Densitometric quantification of the corresponding blots for cleaved caspase-11 (E), GSDM-D (F), caspase-1 (G), and NLRP3 (H), were related to GAPDH levels and represented as arbitrary units (a.u.). Results are expressed as median with a range of at least three independent samples. Differences between the two groups (wt and Epm2b−/− mice) were analyzed by Mann-Whitney non-parametric t-test. P-values have been considered as *p<0.05 and **p<0.01; ns corresponds to no significant differences. (I), Representative confocal images of the immunofluorescence analysis of the RLM+MoDG area from 16 months-old Epm2b−/− mice using anti-GFAP (red), anti-Iba1 (magenta), and anti-GSDM-D (green). Nuclei (blue) were stained with DAPI. The scale bar, shown in the merged images (central and right panels), corresponds to 25μm. A squared dashed line indicates the area that is magnified.