Table I:

Top upstream regulators of RNAseq dataset from the brain of LD mice in comparison to wild type, ordered by z-score (z-score >2 was defined as the threshold of significant activation). The p-value and the regulated genes are indicated.

Upstream mediator	z-score	p-value	Regulated genes
TNF	5.07	2.11 E-17	ARG1, C3, CASP4, CCL2, CCL5, CCN5, CST7, CXCL6, CXCL10, IL1RL1, ITGAX, LCN2, LGALS3, MMP12, MMP3, STEAP4, TIMP1
IFNgamma	4.61	1.52 E-31	ARG1, C3, CCL2, CCL5, CCL8, CD74, CHIL4, CXCL6, CXCL10, FCGR3A, HCAR2, IL1RL1, ITGAX, LCN2, MMP3, MMP12, RENTLA, TGM1, TIMP1
IL1beta	4.51	1.84 E-17	ARG1, C3, CASP4, CCL2, CCL5, CCL17, CD74, CXCL6, CXCL10, FCGR2B, IL1RL1, LCN2, MMP12, MMP3, TGM1, TIMP1, TNFRSF9
CSF2	4.40	4.34 E-19	ACP5, ARG1, C3, C4A, CLEC7A, CCL2, CCL5, CCL17, CD74, CHIL4, CXCL6, CXCL10, FCGR3A, GPNMB, IL1RL1, ITGAX, MMP12, RENTLA, TNFSF8
NFkB	4.23	6.23 E-15	BCL2A1, C3, CCL2, CASP4, CCL5, CD74, CXCL6, CXCL10, IL1B, LCN2, RENTLA, TNFRS9
IL33	4.11	4.32 E-25	CCL2, CCL5, CCL11, CCL17, CHIL4, CXCL10, IL1RL1, MMP12, TIMP1, TNFRSF9
IL1alpha	3.76	1.04 E-14	ACP5, C3, CCL2, CCL5, CXCL6, CXCL10, LCN2, MMP3, MMP12, TIMP1
IL6	3.74	2.01 E-16	ACP5, ARG1, C3, CCL2, CCL5, CD74, CHIL4, CXCL6, CXCL10, HLA-DQA1, HLA-DRB5, IL1RL1, LCN2, LYZ, MMP3, MMP12, RENTLA, STEAP4, TIMP1