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. 2023 Nov 27;14:7770. doi: 10.1038/s41467-023-43652-x

Fig. 8. Relative abundance and α-diversity of gut microbiota significant taxa from immunocompetent and immunodeficient mouse models.

Fig. 8

16S rRNA gene analysis was performed in faecal samples at 6, 12, and 20 weeks of age of different mouse strains: control C57BL/6J (6 weeks: n = 10, 12 weeks: n = 10, 20 weeks: n = 9), isolated NOD or isoNOD (6 weeks: n = 25, 12 weeks: n = 23, 20 weeks: n = 12), cohoused NOD or coNOD (6 weeks: n = 21, 12 weeks: n = 22, 20 weeks: n = 10), isolated 116C-NOD or iso116C-NOD (6 weeks: n = 21, 12 weeks: n = 21, 20 weeks: n = 12), NOD.RAG-2−/− (6 weeks: n = 8, 12 weeks: n = 8, 20 weeks: n = 8), and 116C-NOD.RAG-2−/− (6 weeks: n = 7, 12 weeks: n = 7, 20 weeks: n = 7). a Relative abundance of significant taxa altered by the autoimmune diabetes lymphocyte milieu. b Relative abundance of segmented filamentous bacteria before (pre-transfer, 6 weeks) and after the transfer (post-transfer, 12 weeks) of total NOD spleen (n = 6), NOD B cells (n = 7), NOD T cells (n = 7), and control NOD.Rag2−/− spleen (n = 4). c α-diversity based on Chao1 and Shannon indexes. d Relative abundance of bacteria affected by 116C-NOD B cells. a, c, and d share the same legend. Data are expressed as mean ± SE. Relative abundance data were analysed using the MaAsLin2 statistical framework (mixed-effects linear regression model, two-sided test, adjustment for multiple comparisons), where p-values were corrected using the false discovery rate (FDR). Chao1 and Shannon indexes were analysed with Mann–Whitney’s test (two-tailed p-values).