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. 2020 Nov 3;133(21):jcs246793. doi: 10.1242/jcs.246793

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Fig. 2. The epidermal periodic patterns reorganize through synchronized stripe duplication. (A) Immunostaining of apical (green, anti-MUP-4) and basal (red, anti-LET-805) CeHDs in L1–L4 larvae and adults. Arrows point to dividing stripes. See also Fig. S2. (B) Quantification of the total numbers of CeHD stripes and body lengths in the embryo, L1–L4 larvae and adults stages (mean±s.e.m., n=20 biological samples/stage). (C) Quantification of the total numbers of CeHD stripes and body lengths of wild-type (WT), sma-6(wk7) and lon-2(e678) mutants at the adult stage (mean±s.e.m., n=20 biological samples/genotype). (D) Double-labeling of microtubules (green, anti-tubulin) and CeHDs (red, anti-MUP-4) in L1–L4 larvae and adults. Arrows point to dividing stripes and nascent microtubule bundles. (E) Double-labeling of α-spectrin (green, SPC-1::GFP) and CeHDs (red, LET-805::RFP) in L1–L4 larvae and adults. Boxed areas in A, D, E are enlarged on the right. ***P<0.001; ns, not significant [Tukey's multiple comparison test (B), unpaired t-test (C)]. Scale bars: 5 μm. — The epidermal periodic patterns reorganize through synchronized stripe duplication. (A) Immunostaining of apical (green, anti-MUP-4) and basal (red, anti-LET-805) CeHDs in L1–L4 larvae and adults. Arrows point to dividing stripes. See also Fig. S2. (B) Quantification of the total numbers of CeHD stripes and body lengths in the embryo, L1–L4 larvae and adults stages (mean±s.e.m., n=20 biological samples/stage). (C) Quantification of the total numbers of CeHD stripes and body lengths of wild-type (WT), sma-6(wk7) and lon-2(e678) mutants at the adult stage (mean±s.e.m., n=20 biological samples/genotype). (D) Double-labeling of microtubules (green, anti-tubulin) and CeHDs (red, anti-MUP-4) in L1–L4 larvae and adults. Arrows point to dividing stripes and nascent microtubule bundles. (E) Double-labeling of α-spectrin (green, SPC-1::GFP) and CeHDs (red, LET-805::RFP) in L1–L4 larvae and adults. Boxed areas in A, D, E are enlarged on the right. ***P<0.001; ns, not significant [Tukey's multiple comparison test (B), unpaired t-test (C)]. Scale bars: 5 μm.