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. 2020 Nov 3;133(21):jcs246793. doi: 10.1242/jcs.246793

Fig. 4.

Fig. 4. The apical CeHDs utilize physical links and extension force to drive stripe duplication process. (A) Relative positions of old CeHD units labeled by MUP-4::mEosFP (red) through whole-body photoconversion, and newly formed CeHD units labeled by unconverted MUP-4::mEosFP (green) during stripe separation. Boxed areas are enlarged below. (B) Measurements of the CeHD stripe thickness and the distance between adjacent stripes at the stages of L1–L4 larvae and young adults (mean±s.e.m., n=6 biological samples/stage). (C) MUP-4::GFP labeling showing the different positions of the CeHD stripes within the same L3 larva at regions of straight versus bent body posture. Boxed areas are enlarged on the right. Arrows point to fissures within the same stripe doublet. (D) Measurements of the distance within stripe doublets and between adjacent stripe doublets at regions of straight versus bent body posture (mean±s.e.m., n=6 biological samples/stage). (E) Bright-field images showing the ventral coiler phenotype caused by inactivation of dorsal body wall muscles in transgenic animals expressing Tetanus toxin under the control of the unc-129 promoter. (F) Desynchronized stripe duplication between dorsal and ventral (D↔V) CeHDs (MUP-4::GFP) caused by excessive extension of the dorsal epidermis in transgenic animals expressing Tetanus toxin under the control of the unc-129 promoter. *P<0.05; ns, not significant (unpaired t-test). Scale bars: 5 μm (A,C,F), 100 μm (E).

The apical CeHDs utilize physical links and extension force to drive stripe duplication process. (A) Relative positions of old CeHD units labeled by MUP-4::mEosFP (red) through whole-body photoconversion, and newly formed CeHD units labeled by unconverted MUP-4::mEosFP (green) during stripe separation. Boxed areas are enlarged below. (B) Measurements of the CeHD stripe thickness and the distance between adjacent stripes at the stages of L1–L4 larvae and young adults (mean±s.e.m., n=6 biological samples/stage). (C) MUP-4::GFP labeling showing the different positions of the CeHD stripes within the same L3 larva at regions of straight versus bent body posture. Boxed areas are enlarged on the right. Arrows point to fissures within the same stripe doublet. (D) Measurements of the distance within stripe doublets and between adjacent stripe doublets at regions of straight versus bent body posture (mean±s.e.m., n=6 biological samples/stage). (E) Bright-field images showing the ventral coiler phenotype caused by inactivation of dorsal body wall muscles in transgenic animals expressing Tetanus toxin under the control of the unc-129 promoter. (F) Desynchronized stripe duplication between dorsal and ventral (D↔V) CeHDs (MUP-4::GFP) caused by excessive extension of the dorsal epidermis in transgenic animals expressing Tetanus toxin under the control of the unc-129 promoter. *P<0.05; ns, not significant (unpaired t-test). Scale bars: 5 μm (A,C,F), 100 μm (E).