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. 2020 Nov 3;133(21):jcs246793. doi: 10.1242/jcs.246793

Fig. 5.

Fig. 5. CeHD stripe duplication requires the functions of apical extracellular collagens. (A) MUP-4::GFP localization in young adult epidermis of wild-type and dpy-2(e8), dpy-7(e88), dpy-10(e128) mutants. (B) Double-labeling of MUP-4::GFP and DPY-2::mCherry in L2–L4 larvae and adults. Arrows point to nascent DPY-2 stripes assembling in the already established CeHD gaps. (C) MUP-4::GFP localization in wild-type and dpy-2(e8) mutant embryos. (D) Variation of the stripe positions in wild-type and dpy-2(e8) embryos represented by the difference between the maximum and minimum distance between adjacent stripes in each embryo (mean±s.e.m., n=10 biological samples/genotype). (E) MUP-4::GFP localization in wild-type and dpy-2(e8) mutant at the L1, L2 and L3 stage. (F) Localization patterns of full-length MUP-4 (MUP-4::GFP) and MUP-4 without the collagen-binding domain (MUP-4::△CBD::GFP) in young adult epidermis in a mup-4(mg36) mutant background. Boxed areas are enlarged below. ***P<0.001 (unpaired t-test). Scale bars: 5 μm (A,C,E,F), 2 μm (B).

CeHD stripe duplication requires the functions of apical extracellular collagens. (A) MUP-4::GFP localization in young adult epidermis of wild-type and dpy-2(e8), dpy-7(e88), dpy-10(e128) mutants. (B) Double-labeling of MUP-4::GFP and DPY-2::mCherry in L2–L4 larvae and adults. Arrows point to nascent DPY-2 stripes assembling in the already established CeHD gaps. (C) MUP-4::GFP localization in wild-type and dpy-2(e8) mutant embryos. (D) Variation of the stripe positions in wild-type and dpy-2(e8) embryos represented by the difference between the maximum and minimum distance between adjacent stripes in each embryo (mean±s.e.m., n=10 biological samples/genotype). (E) MUP-4::GFP localization in wild-type and dpy-2(e8) mutant at the L1, L2 and L3 stage. (F) Localization patterns of full-length MUP-4 (MUP-4::GFP) and MUP-4 without the collagen-binding domain (MUP-4::△CBD::GFP) in young adult epidermis in a mup-4(mg36) mutant background. Boxed areas are enlarged below. ***P<0.001 (unpaired t-test). Scale bars: 5 μm (A,C,E,F), 2 μm (B).