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. 2019 Dec 18;133(5):jcs237610. doi: 10.1242/jcs.237610

Fig. 2.

Fig. 2. Tunicamycin and brefeldin A treatment decreased the Ca2+ currents in murine macrophages. (A,C,E,G) Ca2+ tracing was performed in Raw 264.7 cells following treatment with 10 μM Tuni for 6 h (A) or 12 h (C), or 10 μM BFA for 6 h (E) or 12 h (G). Representative traces of the analog plots of the fluorescence ratio (340/380) from an average of 40–60 cells are shown. (B,D,F,H) Quantification (mean±s.d.) of fluorescence ratio (340/380) under conditions as shown in A,C,E,G, as labeled in the figure. (I) Whole-cell patch recording showed that bath application of 1 µM Tg induced an inward-rectifying current in Raw 264.7 cells. (J,K) Average IV curves (J) and current density (K) from 6–9 cells at −80 mV under conditions as shown in I. *P<0.05 by one-way ANOVA test; NS, non-significant.

Tunicamycin and brefeldin A treatment decreased the Ca2+ currents in murine macrophages. (A,C,E,G) Ca2+ tracing was performed in Raw 264.7 cells following treatment with 10 μM Tuni for 6 h (A) or 12 h (C), or 10 μM BFA for 6 h (E) or 12 h (G). Representative traces of the analog plots of the fluorescence ratio (340/380) from an average of 40–60 cells are shown. (B,D,F,H) Quantification (mean±s.d.) of fluorescence ratio (340/380) under conditions as shown in A,C,E,G, as labeled in the figure. (I) Whole-cell patch recording showed that bath application of 1 µM Tg induced an inward-rectifying current in Raw 264.7 cells. (J,K) Average IV curves (J) and current density (K) from 6–9 cells at −80 mV under conditions as shown in I. *P<0.05 by one-way ANOVA test; NS, non-significant.