Figure 2. Identification of human Schwann cell (hSC) cultures using distinct antibody combinations.

Low (A–B) and high (C–G) magnification images of typical hSC cultures are shown to reveal the reactivity and specificity of some recommended antibody combinations. Cultures containing a proportion of non-glial cells (white arrowheads) were selected for display. The morphology of hSCs is varied according to the plating density, the formulation of the culture medium, and the level of fibroblast contamination, but the antibody reactivity and specificity are unchanged. All cultures were grown in HP medium except for those in panel G, which were grown in differentiation medium for seven days (Protocol 3C). Refer to Table 1 for technical details and interpretation of results. Nuclei were stained with DAPI (blue) in all images.