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hSCs were plated, deprived of mitogenic factors and serum, and stimulated as per Protocol 3A. The cells were stained with antibodies against the phosphorylated (P-ERK) and total forms of ERK, respectively. hSCs rapidly respond to β1-heregulin by inducing P-ERK and its shuttling to the nucleus. ERK staining is strong and homogeneous in the hSC’s cytoplasm (right panels, control).
