Video 1. Human Schwann cells (hSCs) deprived of mitogenic factors.

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hSCs were plated on a PLL-laminin-coated dish in LP medium and imaged using IncuCyte ZOOM^TM using 20× objective lenses. This condition serves as a common control for the proliferation (Video 2) and differentiation (Video 3) assays. Notice the fast adhesion of the cells, the changes in cell morphology, and the progressive cell-cell alignment. hSCs proliferated moderately (see mitotic figures) due to serum factors present in the LP medium. Individual phase contrast images were taken every 30 min starting 4 h post-plating. Some relevant elements were highlighted in the video.