Video 3. Human Schwann cell (hSC) differentiation in response to CPT-cAMP.
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Cells were plated in LP medium for 4 h before being stimulated with CPT-cAMP (250 µM) per (Protocol 3C). The plates were transferred to IncuCyte ZOOMTM for live cell imaging immediately after CPT-cAMP stimulation. Notice that CPT-cAMP inhibits hSC proliferation, alignment, and migration while inducing a flat, expanded cytoplasm that features transient vacuoles of different sizes. This culture is ready to be analyzed for the presence of myelin-related markers by the end of the incubation period. Some relevant elements were highlighted in the video. For a reference, compare this video with Video 1 (control condition).